Equipment: Tissue Clearing and Preparation

Choosing the best clearing method for your purposes requires the careful consideration of several factors. Below are some resources for learning more about the two principal clearing methods compatible with our microscopes.

iDISCO

The following are useful resources for learning about and using the iDISCO method of tissue clearing and preservation. This is an organic solvent based clearing method.

iDISCO
This site is a useful resource for organic solvent based clearing.

iDISCO Protocol – iDISCO Method
A Basic protocol for iDISCO clearing.

Validated Antibodies – iDISCO Method
A list of antibodies that have been validated to work with organic based solvents.

DISCO+ Protocol with Tips and Tricks
iDISCO+ Protocol Checklist
These two documents were generated at Richard Simerly’s Lab. They are modified from the 2016 idisco protocol in the first point here.

SHIELD

The following are useful resources for learning about and using the SHIELD method of tissue clearing and preservation. This is an aqueous based clearing method.

SHIELD — Chung Lab Resources
A useful resource for learning about and using the SHIELD method of tissue preparation.

Chung Lab’s Protocol for Clearing

Instructions for Sample Mounting for Imaging in Our SMARTSPIM